Antihemolytic activity of hydroalcoholic leaves and bark extracts from Rhamnus alaternus against AAPH induced hemolysis on human erythrocytes

Rhamnus alaternus is a Mediterranean shrub that has been used in traditional medicine to treat various diseases. This study aimed determine the phenolic composition, as well antioxidant and antihemolytic activities of R. leaves (LRA) bark (BRA), extracts against AAPH-induced hemolysis. The extraction yields were 19.8% 18.2% for bark. Total polyphenols (88.1 ± 1.83 mg GAE/g) condensed tannins (36.24 5.44 CE/g) higher BRA extract than LRA (80.22 1.4 GAE/g 23.48 0.25 CE/g, respectively). However, was found be richer total flavonoids (64.6 2.6 QE/g) flavones/flavonol (18.34 1.65 (39.87 ±0.58 QE/g 10.08 0.35 QE/g), respectively. IC50 DPPH ABTS radical scavenging activity 86.59 2 µg/ml 12.49 0.29 69.23 2.14 12.83 0.13 extract, Also, both showed good reducing power with 157.09 5.53 Asc E/g 194.97 1.46 extract. hemolytic effect tested on human erythrocytes, did not have cytotoxic effects at low doses. To induce hemolysis, AAPH concentration 200 mM an incubation time 4h. two pretreatment erythrocytes doses significantly reduced hemolysis dose-dependent manner. Indeed, µg/ml, percentages inhibition 99.41 1.17% 76.26 12.03% extracts, more effective (IC50 = 106.70 1.48 µg/ml) compared 148.64 7.04 µg/ml). Our results demonstrate first attenuates can prevent anemias.